Chondrogenic potential of adipose-derived stem cells versus bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2013.32.008

Abstract

Abstract:

BACKGROUND: Adipose-derived stem cells and bone marrow mesenchymal stem cells are used widely in cartilage tissue engineering, and there are many similarities in biological characteristics between two kinds of cells.
OBJECTIVE: To compare the chondrogenic potential of bone marrow mesenchymal stem cells and adipose-derived stem cells in vitro.
METHODS: Adipose-derived stem cells were isolated from the 3-month-old New Zealand white rabbits’ abdomen. Bilateral femurs of rabbits were obtained, and then the bone marrow mesenchymal stem cells were separated with the adherence screening method. The growth curve of the passage 3 adipose-derived stem cells and bone marrow mesenchymal stem cells were drawn, and the doubling time of two kinds of cells was compared. Then the passage 3 adipose-derived stem cells and bone marrow mesenchymal stem cells were treated with chondrogenic induction. After induced for 14 days, the adipose-derived stem cells and bone marrow mesenchymal stem cells were treated with toluidine blue staining and type Ⅱ immunohistochemistry staining respectively.
RESULTS AND CONCLUSION: Primary bone marrow mesenchymal stem cells showed aggregative growth, while the primary adipose-derived stem cells were in single and scattered growth. The proliferation speed of adipose-derived stem cells was faster than that of bone marrow mesenchymal stem cells, while the doubling time of adipose-derived stem cells was shorter than that of the bone marrow mesenchymal stem cells. After chondrogenic induction for 14 days, both adipose-derived stem cells and bone marrow mesenchymal stem cells could express glycosaminoglycans and type Ⅱ collagen, and the expression level of type Ⅱ collagen in bone marrow mesenchymal stem cells after chondrogenic induction was higher than that in the adipose-derived stem cells. The in vitro proliferation of adipose-derived stem cells and bone marrow mesenchymal stem cells was rapid and stable, but the proliferative ability of adipose-derived stem cells was faster than that of bone marrow mesenchymal stem cells. When cultured in single layer, both adipose-derived stem cells and bone marrow mesenchymal stem cells could transform into chondrocytes under certain conditions, but bone marrow mesenchymal stem cells seemed to be more potential than adipose-derived stem cells.

Key words: stem cells, adipose-derived stem cells, bone marrow mesenchymal stem cells, bone marrow, adipose tissue, chondrogenic differentiation, type Ⅱ collagen, mesenchymal stem cells, provincial grant-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

An Rong-ze, Zhao Jun-yan, Wang Zhao-jie . Chondrogenic potential of adipose-derived stem cells versus bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(32): 5793-5798.

Figures/Tables 3

References

[1] Poulsom R, Forbes SJ, Hodivala-Dilke K, et al. Bone marrow contributes to renal parenchymal turnover and regeneration. J Pathol. 2001;195(2):229-235.

[2] Nayyer L, Patel KH, Esmaeili A, et al. Tissue engineering: revolution and challenge in auricular cartilage reconstruction. Plast Reconstr Surg. 2012;129(5):1123-1137.

[3] Morigi M, Imberti B, Zoja C, et al. Mesenchymal stem cells are renotropic, helping to repair the kidney and improve function in acute renal failure. J Am Soc Nephrol. 2004;15(7):1794-1804.

[4] Hamid AA, Idrus RB, Saim AB, et al. Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation. Clinics (Sao Paulo). 2012;67(2):99-106.

[5] Mödder UI, Roforth MM, Nicks KM, et al. Characterization of mesenchymal progenitor cells isolated from human bone marrow by negative selection. Bone. 2012;50(3):804-810.

[6] Vishnubalaji R, Al-Nbaheen M, Kadalmani B, et al. Comparative investigation of the differentiation capability of bone-marrow- and adipose-derived mesenchymal stem cells by qualitative and quantitative analysis. Cell Tissue Res. 2012; 347(2):419-427.

[7] 杨中萌,尹震宇,叶志坚,等.大鼠脂肪和骨髓来源间充质干细胞的生物学特性比较[J].中国组织工程研究与临床康复,2010,14(23): 4211-4216.

[8] 安荣泽,王兆杰,张强,等.改良诱导方案诱导SD大鼠脂肪源性干细胞定向分化为软骨细胞[J].中国组织工程研究与临床康复, 2009,13(36):7052-7057.

[9] Mithoefer K, Williams RJ 3rd, Warren RF, et al. The microfracture technique for the treatment of articular cartilage lesions in the knee. A prospective cohort study. J Bone Joint Surg Am. 2005;87(9):1911-1920.

[10] Lee RH, Kim B, Choi I, et al. Characterization and expression analysis of mesenchymal stem cells from human bone marrow and adipose tissue. Cell Physiol Biochem. 2004; 14(4-6):311-324.

[11] Strem BM, Hicok KC, Zhu M, et al. Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med. 2005;54(3):132-141.

[12] Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7(2):211-228.

[13] Gronthos S, Franklin DM, Leddy HA, et al. Surface protein characterization of human adipose tissue-derived stromal cells. J Cell Physiol. 2001;189(1):54-63.

[14] Baksh D, Yao R, Tuan RS. Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow. Stem Cells. 2007;25(6):1384-1392.

[15] 王兆杰,安荣泽,张强,等.利用脂肪干细胞构建软骨修复兔膝关节软骨缺损的初步研究[J].中国骨质疏松杂志,2009,15(7): 501-505.

[16] 杨震,简月奎,安荣泽,等.SD大鼠脂肪干细胞的体外分离培养及实验研究[J].贵州医学,2008,32(5):54-58.

[17] 李松军,安荣泽,谢伟.TGF-β1和bFGFs体外诱导成人骨髓间充质干细胞表达软骨细胞表型的实验研究[J].中国矫形外科杂志. 2005,13(5):608-611.

[18] 王兆杰,安荣泽,袁小洪.重组腺相关病毒介导转化生长因子-β1基因修饰ADSCs成软骨细胞的研究[J].中华实验外科杂志,2010, 27(12);1958-1958.

[19] 中华人民共和国科学技术部. 关于善待实验动物的指导性意见.2006-09-30.

[20] 刘坤,罗军,娄繁,等.大鼠骨髓基质细胞的体外培养和鉴定[J].中国普外基础与临床杂志,2009,16(11):875-879.

[21] 黄谦,吴涛,梁杰,等.全骨髓培养法和密度梯度离心法分离hBMSCs的比较研究[J].中国修复重建外科杂志,2009, 23(11): 1360-1364.

[22] Rebelatto CK, Aguiar AM, Moretão MP, et al. Dissimilar differentiation of mesenchymal stem cells from bone marrow, umbilical cord blood, and adipose tissue. Exp Biol Med (Maywood). 2008;233(7):901-913.

[23] 余方圆,卢世璧,赵斌,等.脂肪干细胞与骨髓干细胞生物学性状的比较[J].中华实验外科杂志,2009,26(3):396.

[24] Winter A, Breit S, Parsch D, et al. Cartilage-like gene expression in differentiated human stem cell spheroids: a comparison of bone marrow-derived and adipose tissue-derived stromal cells. Arthritis Rheum. 2003;48(2): 418-429.